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Image Search Results
Journal: Biomatter
Article Title: Comparison of the reaction of bone-derived cells to enhanced MgCl 2 -salt concentrations
doi: 10.4161/21592527.2014.967616
Figure Lengend Snippet: Cell proliferation. Cell count ( A ), viability ( B ) and division and proliferation rate ( C ) of the osteosarcoma derived cell lines U2OS, MG63 and SaoS2 and osteoblasts (OB) after incubation with increasing concentrations of MgCl 2 (0-25 mM). Significant differences between control and indicated conditions are presented by asterisks or hash marks ( P < 0.05 = *, P < 0.001 = #). The viability is normalized to the total amount of cells measured in ( A ).
Article Snippet: We wish to thank Gabriele Salamon and
Techniques: Cell Counting, Derivative Assay, Incubation, Control
Journal: Biomatter
Article Title: Comparison of the reaction of bone-derived cells to enhanced MgCl 2 -salt concentrations
doi: 10.4161/21592527.2014.967616
Figure Lengend Snippet: Cell size of trypsinised cells in suspension ( A ) and adherent cells on fibronectin coated glass slides ( B ) of the osteosarcoma derived cell lines U2OS, MG63 and SaoS2 and osteoblasts (OB) after incubation with increasing extracellular MgCl 2 concentrations (0-25 mM). Significant differences between the control and indicated conditions are presented by asterisks or hash marks ( P < 0.05 = *, P < 0.001 = #).
Article Snippet: We wish to thank Gabriele Salamon and
Techniques: Suspension, Derivative Assay, Incubation, Control
Journal: Biomatter
Article Title: Comparison of the reaction of bone-derived cells to enhanced MgCl 2 -salt concentrations
doi: 10.4161/21592527.2014.967616
Figure Lengend Snippet: Cell size of adherent cells on fibronectin coated glass slides. Fluorescent microscopy of U2OS cells under cell culture conditions ( A ) and after addition of 25 mM MgCl 2 ( B ), MG63 ( C and D ), SaoS2 ( E and F ) and osteoblasts ( G and H ), respectively. Actin filaments were stained green, and the nuclei were stained blue. The scales show a length of 50 μm, except the scale in H, where the length is 100 μm.
Article Snippet: We wish to thank Gabriele Salamon and
Techniques: Microscopy, Cell Culture, Staining
Journal: Biomatter
Article Title: Comparison of the reaction of bone-derived cells to enhanced MgCl 2 -salt concentrations
doi: 10.4161/21592527.2014.967616
Figure Lengend Snippet: Semi-quantitative gene expression. Gene expression of genes involved in bone metabolism from osteosarcoma-derived cell lines U2OS ( A ), MG63 ( B ), SaoS2 ( C ) and osteoblasts ( D ) after incubation with various MgCl 2 concentrations (0-25 mM). Gene expression was normalized to the expression of GAPDH and ß-Actin. Expression of 0 mM MgCl 2 was set as the control (= 1). GAPDH: glyceraldehyde 3-phosphate dehydrogenase; ALP: alkaline phosphatase; BSP: bone sialoprotein; OPN: osteopontin; OPG: osteoprotegerin; RANKL: RANK ligand. Significant differences between the control and indicated conditions are presented by asterisks or hash marks ( P < 0.05 = *, P < 0.001 = #).
Article Snippet: We wish to thank Gabriele Salamon and
Techniques: Gene Expression, Derivative Assay, Incubation, Expressing, Control